ZetaView

The ZetaView® is a Nanoparticle Tracking Analysis (NTA) instrument for measuring hydrodynamic particle size, zeta potential and concentration. Now also available with TWIN lasers, for enhanced florescence measurement capability.

Key Features:

  • Scanning NTA – measurements are taken at 11 different positions in the sample cell. Gives a more representative analysis than single-point measurements
  • Auto-alignment and auto-focus – the optical setup is automatically optimised by the software, saving the user time preparing the instrument for use and removing subjective user input bias
  • Fast measurements – size and concentration readings can be achieved in as little as 90 seconds (based on 11 positions, 2 cycles)
  • Quick and simple cleaning – cleaning between samples requires only a quick flush (less than 1 minute), again benefiting the user by saving time
  • Intuitive software – traffic-light systems give an instant indication of whether the sample is at a suitable concentration for measurement. Gives the user an indication of what the correct dilution factor of their samples is
  • Compact all-in-one design – highly compatible with the laboratory environment; small footprint and data files
  • Discrimination power – by fluorescence measurements, allowing specific detection of sub-populations
  • Twin laser model available – the ZetaView is now available as a twin-laser model, giving the user a choice of two lasers to use for fluorescent labeling and excitations. Two different flourophores can be used on the same sample, for identification of TWO different sub-populations

As exclusive distributor for the UK and Ireland, Analytik is proud to supply the Zetaview® from Particle Metrix. Through Analytik, you’ll have access to our team of experts to help develop your application and get the most out of your investment.

For more information, or to discuss your requirements with a product specialist, please call us on +44 (0)1954 232 776 or email info@analytik.co.uk.

Description

Nanoparticle Tracking Analysis with the ZetaView 

The ZetaView® is a nanoparticle tracking analysis (NTA) instrument for measuring hydrodynamic particle size, zeta potential and concentration.

With nanoparticle tracking analysis, you measure what you see. Each individual particle in the field of view is counted and tracked in short video clips, creating accurate concentration calculations and particle size distributions. The ZetaView® combines these measurements with classical micro-electrophoresis, for determination of zeta-potential. All of these parameters can be measured both in scattering mode and in fluorescence mode, for determination of fluorescently-labelled sub-populations. The result is one compact instrument capable of measuring size, concentration, zeta-potential and fluorescence.

The main advantage of the ZetaView® system is that the optical setup can be moved. The laser and microscope objective can be moved both synchronously and independently.Optical Layout of the automated particle tracking ZetaView® Laser Light Scattering unit

  • Moving the laser and objective synchronously allows sub-volume scanning of the sample cell. Measurements can be taken at 11 different positions across the cell, meaning measurements are based on having tracked thousands of individual particles, giving a more representative analysis than single-point measurements, and adding more power to the statistical analyses.
  • Moving the optics independently allows the system to automatically align the laser and objective, therefore automatically focussing the image of the particles and significantly minimising setup time. Once the the focussing is done, there is no need to repeat the procedure for the rest of the day.

qualityZeta Potential

Zeta potential is effectively a measure of the surface charge of a particle and the ZetaView® uses micro-electrophoresis to calculate it.

The zeta potential distribution is calculated from the electrophoretic mobility results at the two stationary layers in the sample cell, or from an electrokinetic velocity profile obtained by scanning at all 11 positions throughout the sample cell.

The zeta-potential is calculated from the Smoluchowski or Henry equation – see the “Theory” tab, above. In addition to the particle zeta potential result, the curvature of a profile delivers information on the polarity and amount of ionic coating on the cell walls.

Zeta Potential Distributions

The below example highlights the capability of the ZetaView® and zeta potential, as well as the importance of measuring zeta potential and its relationship with hydrodynamic particle size.A: dispersed in 2 mM KCl solution (stable). B: dispersed in distilled water (unstable)

A 60 nm gold dispersion was studied for size and zeta potential distribution (pictures on the right). The sample is slightly bi-modal.

Curve A (red) represents gold particles in a 2m KCl environment, curve B (blue/green) the same sample but dispersed in distilled water.

The zeta potential distribution of curve B shows a peak near 0 mV, which is making the suspension unstable, due to a lack of repulsive force between the particles (no charge). The majority of the 60 nm particles are therefore agglomerated, which is clearly seen in the size distribution of curve B.

ZetaView and Fluorescence (F-NTA)
fluorescence

With NTA, particles are viewed by detection of the light that they scatter. Due to the physical nature of this detection method, a chemical discrimination is not provided. In order to add specificity to particle characterisation, Fluorescent Nanoparticle Tracking Analysis (F-NTA) can be employed.

Nanoparticle such as vesicles can be labelled with a specific antibody bearing a fluorescent molecule (fluorophore). The ZetaView® is fitted with a slide-in filter to block scattered light from the laser, allowing past only fluorescence of a longer wavelength, generated by excitation of the fluorophores by the laser. Therefore, only the fluorescently-labelled particles will be detected and measured by the camera.

NEW Zetaview TWIN Laser

The ZetaView is now available as a Twin Laser model, incorporating two different laser modules and corresponding filters which can all be automatically switched at the click of a button. As well as giving the user a choice of two different lasers to use for their fluorescence measurements, the Twin Laser ZetaView allows detection and characterisation of TWO different fluorescently labelled sub-populations, adding extra layers of specificity to NTA measurements.

The Twin Laser model arrives in the same small, compact, highly-engineered chassis as the standard ZetaView. On top of the twin fluorescence emission detection capabilities, the standard size, concentration and zeta potential capabilities remain, making the Twin Laser ZetaView a completely unique offering and very much the next generation of NTA technology.

Interested in the Zetaview®? Request a quote or call us on +44(0)1954 232 776 to discuss your requirements.

Theory

Particle SizeParticle size by Brownian motion: Nanoparticle Tracking Analysis (NTA)

Calculation of hydrodynamic particle size is achieved by analysis of short video clips of particles moving in a liquid under Brownian Motion.

Brownian Motion is the random movement of particles in a liquid caused by constant collisions with the fast-moving molecules of the containing liquid.

The ZetaView® software analyses video clips to calculate the mean square displacement. The Stokes-Einstein equation is then applied to calculate hydrodynamic particle size, as shown in the graphic, right.

Zeta PotentialPrinciple of zeta potential determination

The ZetaView® uses micro-electrophoresis in order to calculate zeta potential.

An electric field is applied across the sample cell and short video clips are taken of the parrticles moving through the cell according to their surface charge and polarity.

The ZetaView® software then analyses the video clips to determine electrophoretic mobility. The Henry/Smoluchowski equation is then applied in order to calculate zeta potential, as shown in the graphic, right.

Applications

  • Extracellular Vesicles (EV) and Exosomes
  • Bio-nanoparticles
  • Liposomes and Micelles
  • Drug Delivery
  • Protein Agglomeration
  • Viruses
  • Virus Like Particles (VLP)
  • Emulsions
  • Polymers
  • Nanometals
  • Nanobubbles
  • Humic Acids
  • Quantum Dots
  • F-lablled Nanoparticles

Videos

Nanoparticle Tracking Analysis (NTA) with ZetaView®

Brownian Diffusion in the Laser Scattering Video Microscope

ZetaView Measurement Principle

120 nm Al203 particles and agglomerates in the electrophoresis set-up of ZetaView

Webinars

Particle Tracking Analysis (PTA) with ZetaView®

Datasheet

PDF Icon ZetaView® Nanoparticle Tracking Analyser Datasheet

PDF Icon ZetaView® TWIN Laser Nanoparticle Tracking Analyser Datasheet

Whitepapers

PDF Icon Introduction to Nanoparticle Tracking Analysis (NTA)

PDF Icon Zeta potential and size measurement of nanoparticles by laser scattering video microscopy

Technical/Application Notes

PDF Icon Three in One: Concentration, Size & Zeta Potential measured on sub-visible protein suspensions

PDF Icon Zeta potential distribution of 1 nm particles

PDF Icon The Scanning Nano Particle Tracking Analysis (NTA) ZetaView™ now for potentially hazardous samples

News & User Stories

News Icon Oxford Brookes University use the Zetaview to study extracellular vesicles

News Icon Cell Guidance Systems use the ZetaView from Particle Metrix for exosome research and quality control

News Icon The Institute for Transfusion Medicine at the University Hospital of Duisburg-Essen in Germany uses the ZetaView from Particle Metrix to quantify extracellular vesicles

News Icon The Translational Research Center at the University Hospital of Erlangen in Germany uses the ZetaView from Particle Metrix to quantify extracellular vesicles such as exosomes

News Icon The Ottawa Hospital Research Institute uses the ZetaView from Particle Metrix to study membrane microparticles as potential biomarkers for underlying diseases

News Icon An Innovative Method for Exosome Quantification and Size Measurement

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