Solutions for Cell Disruption

Cell disruption is the process of breaking cell wall and/or membrane to release intracellular fluids containing molecules or particles of interest, such as proteins or viruses. The primary goal of cell disruption is to lyse the suspended cells and recover the maximum possible viable yield of the molecules/particles of interest.

Microfluidizer® high-shear fluid processors are ideally suited to rupturing various cell types, including bacterial (gram positive and gram negative), yeasts, insect, mammalian, plant and algae cells, due to the wide and controlled shear rate range they can apply to the sample. This control of the shear rate enables researchers to optimise the process parameters to achieve effective rupture rates while minimising the excess energy introduction to the system and avoiding denaturation of delicate proteins.

Microfluidizer® high-pressure processors (or cell disruptors) offer a number of advantages over other methods of homogenisation, including High Pressure Homogenisers. View an application note which provides an overview of cell disruption techniques and why Microfluidizer® processors are best suited for cell disruption.

Browse Solutions for Cell Disruption below; call us on +44(0)1954 232 776 or email info@analytik.co.uk to discuss your requirements with a specialist.

Cell Disruptors

The LM20 is an ideal Microfluidizer for cell disruption; digitally controlled, it has a maximum process pressure of 30,000 psi enabling it to handle the toughest cell types.

LM20 Microfluidizer High Pressure Homogeniser

The LM10 enables extremely fast, easy and consistent processing of all cell suspensions. Digital control enables repeatability between sample batches and simple operation.

The LM10 Microfluidizer® high pressure lab homogeniser

The above Microfluidizer models are the most popular for cell disruption amongst our customers’ applications, however other Microfluidizers are available.

Bacteria

Microfluidizers handle disruption of E.Coli and other bacterial cell lines easily. Typically sufficient lysis is achieved in 1 pass at moderate process pressures, minimizing excess energy and heat in the system and enabling excellent recovery of viable protein.

 

Microfluidizer® Processor Laboratory Results

Industry Biotechnology/Pharmaceutical
Product E.Coli suspension
Test Objective Achieve a high degree of cell disruption
Processor M-110EH
Chambers • Interaction Chamber (downstream) H10Z
• Auxiliary Processing Module (upstream) H30Z
No. of Passes 1
Shear per Pass 4,800,000 sec-1
Process Pressure 18,000 psi (1241 bar)
Before Processing
(Magnifi cation 1000 X,
1 division=1 micron)
Microfluidizer for Cell Disruption - E.coli: Before Processing
1 Pass
(Magnifi cation 1000 X,
1 division=1 micron)
Microfluidizer for Cell Disruption - E.coli: 1 Pass
Summary of Results Estimated that greater than 95% cell disruption was achieved after 1 pass at 18,000 psi using an M-110EH Microfluidizer® materials processor.

Contact us via info@analytik.co.uk or call +44 (0)1954 232 776 to discuss your requirements.

Yeast

Yeast cell types are typically resilient by comparison to other bacteria and insect cells. Microfluidizer’s exceptionally high and homogenous shear rate and control of process temperature makes them ideal for processing of the most challenging Yeast species where multiple passes may be required.

 

Microfluidizer® Processor Laboratory Results

Industry Biotechnology/Pharmaceutical
Product Yeast Cell
Test Objective Obtain 90% cell disruption of yeast cells
Processor M-110EH
Chambers • Interaction Chamber (downstream) H10Z
• Auxiliary Processing Module (upstream) H30Z
No. of Passes 5
Shear per Pass 5,780,000 sec-1
Process Pressure 25,000 psi
Before Processing
(Magnifi cation 400 X,
1 division = 2.5 μm)
Microfluidizer for Cell Disruption - Yeast: Before Processing
5 Passes
(Magnifi cation 400 X,
1 division = 2.5 μm)
Microfluidizer for Cell Disruption - Yeast: 5 Passes
Summary of Results Achieved greater than 90% cell disruption after 5 passes using the M-110EH Microfluidizer® materials processor.

Contact us via info@analytik.co.uk or call +44 (0)1954 232 776 to discuss your requirements.

Insect

Insect cells, such as Sf9, are comparatively easy to disrupt compared to Bacteria and Yeasts; Microfluidizers enable fast, consistent processing at moderate pressures usually requiring a single pass for superior lysis.

Contact us via info@analytik.co.uk or call +44 (0)1954 232 776 to discuss your requirements.

Mammalian

The easiest cell type to disrupt; when required processing of these cell needs to be handled gently to preserve delicate viruses or proteins. Mild conditions are used and scalability and easy downstream filtration are among the main benefits for using Microfludizer technology for this application.

Contact us via info@analytik.co.uk or call +44 (0)1954 232 776 to discuss your requirements.

Algae

Algae cells such as Diatoms and various other naturally occurring species can be successfully lysed on a Microfluidizer with varying conditions for research, ingredient harvesting or biofuel purposes.

 

Microfluidizer® Processor Laboratory Results

Industry BioTech Company F
Product A 10% wt. dispersion of algae cells suspended in water.
Test Objective To rupture the cells in order to free the oil that is contained inside.
Processor M-110S
Chambers • G10Z (87 microns)
No. of Passes 3
Process Pressure 20,000 psi
Process Procedure The algae cells were suspended in water at a concentration of 15% wt. cells when they arrived.
The original concentration was very viscous, such that it was diluted down to approximately
10% wt. before processing. The samples were processed on the M-110S Microfluidizer
processor for one – three passes at 20,000 psi. A sample was taken after each pass and analysed.
Summary of Results The optical microscopy results from the analysis can be found in Figure 1.

Microfluidizer for Cell Disruption - Algae

Figure 1: Pictured above are optical microscopy images of BioTech Company F’s algae cell dispersion (A) before processing, (B) after one pass, (C) after two passes, and (D) after three passes with the G10Z (87 μm) IXC. All samples were processed with the M-110S Microfluidizer processor at 20,000 psi.

 

Contact us via info@analytik.co.uk or call +44 (0)1954 232 776 to discuss your requirements.

Our customers

The LM20 Microfluidizer processor offers superior lysis over traditional methods of cell disruption with an efficient system to reduce internal blockages. The scalability of the design means that the forces experienced by cells will not change if the sample volume increases. I can, therefore, have confidence that the sample quality, yield and processing times won’t be impacted by scaling up my lab processes.

Digital controls allow the system pressure to be set more easily and accurately compared to alternative manual processors, reducing the potential for errors and increasing reproducibility.

Analytik’s in-depth user training, and easy operation/maintenance procedures have been extremely beneficial. I feel confident to troubleshoot and perform preventative maintenance without having to call the support teams – it doesn’t feel like a ‘black box’ machine.

I would recommend the LM20 Microfluidizer as an essential tool for cell disruption.

James Wright, Protein Scientist at Abcam Plc

We approached Analytik to find a replacement for a high-pressure homogeniser from another manufacturer, which had been unreliable and failed to achieve sufficient lysis of our tough yeast cells even after very many passes. We selected the LM20 Microfluidizer not only because it efficiently lysed a broad range of cell types but also was incredibly simple and easy to use which is very important as many different people need to use the machine regularly.

We also much appreciated the knowledgeable technical and applications support provided by Analytik which has enabled us to fully exploit the considerable capabilities of the LM20 Microfluidizer.

Dr Petra Ungerer, Lab Manager, School of Biological & Chemical Sciences at Queen Mary University London

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