Introducing the ZetaView NTA x30 Instrument Range for Measuring Particle Concentration
ZetaView® is an advanced Nanoparticle Tracking Analyser for measuring hydrodynamic particle size, zeta potential, concentration, fluorescence and colocalization.
Unique Features & Benefits
Scanning NTA
ZetaView® uses unique scanning NTA technology to take automated measurements at 11 positions through the sample cell, providing a thorough interrogation of samples and increased accuracy, without need for additional accessories. The instrument’s 2 internal pumps can be used to ‘push’ a fresh sub-volume of sample in front of the objective. The result?
- Highest sampling volume of any NTA instrument for higher accuracy
- No sample bleaching
- Fast measurements – analyse over 2,000 particles in as little as 60 secs
- Full sample recovery
Auto-alignment and auto-focus
The optical setup is automatically optimised by the system’s advanced, user-friendly software, saving instrument preparation time and, importantly, removing subjective user input bias.
Multi-laser configuration
With a choice of 1, 2 or 4 lasers in the same system configuration and a 12- position filter wheel, the ZetaView easily enables fluorescent labeling and excitation of one, two or four different flourophores on the same sample, for identification of at least ONE, TWO or FOUR different sub-populations.
Ease of Use
ZetaView’s fixed cell assembly means no disruptive disassembly required between sampling. Measure at the touch of a button!
In addition, ZetaView offers best-in-class software with a user-friendly interface, intuitive processes and clear presentation of results, which are easily exported.
Other Benefits
- No high cost consumables – just syringes and reference standards for scattering and fluorescence measurements (if applicable)
- No calibration – size measurements do not need to be calibrated as the method is absolute
- Quick and simple cleaning – cleaning between samples simply requires a quick flush, which takes less than 1 minute!
- Intuitive software – a traffic-light system gives an instant indication of whether the sample is at a suitable concentration for measurement
- Fixed cell assembly design – greatly enhanced performance and less susceptibility to errors
- Compact all-in-one design – compatible with the lab environment small footprint and data files
Ideal Applications
- Bio-nanoparticles:
▫ Extracellular Vesicles (EVs)
▫ Exosomes
▫ Liposomes & Micelles
▫ Protein Agglomerates
▫ Viruses & Virus-Like-Particles (VLPs)
▫ Drug Delivery
▫ Fluorescent-labelled Nanoparticles
- Low Concentration Samples:
▫ Nanobubbles
▫ Trace Particles
▫ Nanometals
▫ Quantum Dots
ZetaView® Colocalization NTA Feature (C-NTA)
Colocalization Nanoparticle Tracking Analysis (C-NTA) is an advanced version of fluorescence-NTA (F-NTA). It not only measures the size and concentration of fluorescently labelled particles but also detects multiple fluorescent targets on a single nanoparticle. Using multiple lasers and specific fluorescence filters, C-NTA can quantify individual fluorescent sub-populations and particles that are double-positive for different surface markers.
ZetaView® uses new generation lasers for precise illumination of NTA channels and quick switching times, combined with smart software, to accurately quantify biomarker colocalization scores. Read more: Colocalization-NTA of multi-stained platelet derived MSC-EV preparations >
Key Features
- Ultra-fast switching between NTA channels
ZetaView’s new SOP manager supports colocalization experiments with quasi-simultaneously two-channel acquisition. The measurement specifications can be edited and stored ensuring maximum traceability.
- Automated diffusion correction
As the diffusion behaviour of the sample is already determined by NTA, results are automatically corrected for the loss as well as emerging of new particles, leading to credible colocalization scores.
- Reliable calculation of colocalization scores
Scatterplots visualize particle matching and statistical information, presented in clear tables.
In a Nutshell
Choose ZetaView® for next-generation NTA with the following benefits you won’t find with any other instrument:
Fastest, most accurate NTA measurements available
Analysis of particles in up to 4 different fluorescence channels
Colocalization for detection of multiple fluorescent targets
No disassembly between samples
Ease of use and cleaning
Best in class analysis software
Fast, effective post-sales and technical support
