Tissue engineering and tissue regeneration have become a promising approach e.g. to cure severe bone injuries. Artificial tissue grown from differentiable cells often needs to be formed in a very particular 3-dimensional shape for implantation. Bio scaffolds can serve as a cell growth environment for such artificial tissues, further supporting supply of the cells and removal of the metabolites. More here.
The BioScaffolder 3.1 is a modular instrument platform with up to four independent z-axes for running several dispensing tools. It can print a variety of pasty materials (hydrogels and biopolymers e.g. collagen, alginate, bone cement paste, polymer paste etc.) with up to three cartridges and has a unique optional heat controlled piezoelectric micro-pipetting system for pico- and nano-litre spotting of cells, proteins and curing/hardening substances on certain coordinates within a scaffold structure.
The BioScaffolder printer has an unique piezoelectric pipetting option which allows non-contact dispensing of small volumes of drops (pico- and nano-litre) typically of cell suspensions or protein solutions on predefined coordinates of a bio-scaffold layer immediately after printing of this layer. The piezoelectric pipettor aspirates from standard 96/384 well plates. Liquid volumes of a few micro-litres work with this system. Also the flexibility of the separate piezoelectric pipettor allows the application of different cells at selected positions of the scaffolds, difficult to achieve by the cartridge dispenser. The dispensed spots usually have sizes between 50 and 200 microns. They can be placed on a scaffold layer immediately after printing and before the next scaffold layer goes on top e.g. particular proteins can be printed or even cell suspensions at very low viscosity.
The combination of both technologies is quite new and unique to the BioScaffolder printer and not available on any other competitive 3D printer.
- Independent printing of scaffold materials and cells/proteins during the scaffold structure printing process.
- Exposition Lamps for Curing and UV-Hardening
- Melt Electrospinning (MES, In development)
- Syringe Extruders (In development)
- GUI-based control software including easily definable cuboids and cylinders. The software is capable
of defining curves and meanders in all three directions and has import function for STL data as part of the new
3D CAD interface capabilities.
- Piezoelectric stroboscope camera: the camera comes as standard with the piezoelectric pipetting option
to check the size/shape of the dispensing spots, allowing the user to change the dispensing parameters
(voltage/frequency) of the piezoelectric tip to fit the sample consistency (viscosity, fluidity, electrical
charge etc.), thus providing an optimised and high spotting performance.
- Up to four independent Z-drives (up to 3 cartridges and 1 optional nano-litre piezoelectric dispenser).
- Nano-litre piezoelectric option allows dispensing of up to 384 sample species on parts of a scaffold
structure in the same printing run without having to stop to change tips or cartridges.
- Heatable cartridge holders (different sizes).
- External control unit for air pressure and fluidics of piezoelectric dispensers.
- Automatic alignment of dispensing capillaries and tip cleaning.
- Printable materials include: hydrogels, biopolymers (e.g. collagen, alginate), bone cement
paste, biocompatible silicones and polymer pastes.
Request a quote for Bio-Scaffolder 3.1 or call us on UK: +44 (0)1954 232 776 / Ireland: +353 (0)144 75170.